Deregulated stress-induced gene expression in SG-defective mutants.

<p>Gene expression analysis for stress-induced (A, B) and stress-repressed genes (C) in wt and SG-defective mutants (<i>mft1Δ</i>, <i>arc1Δ</i>, <i>rps28bΔ</i>, <i>rpl20bΔ</i>, <i>top3Δ</i>) exposed to stress. The degree of induction or repression of stress-responsive genes was determined using qPCR. To normalize, the levels of <i>ACT1</i> mRNA was used as a reference, and final values were represented as a percentage of the maximum value in wt cells as indicated in each graph. Data represent at least two independent experiments. Data points and error bars show mean and standard deviation. Statistical analyses were performed using Student's t-test of comparisons between wt and mutants in the same time point (* p<0.05, ** p<0.01). <b>A</b>) Induction of the <i>HSP12</i> or <i>HSP104</i> transcripts in cells exposed to 400 mM 2-DG. <b>B</b>) As in A, but cells were exposed to heat shock (44°C). <b>C</b>) Repression of the <i>PWP1</i>, <i>UTP13</i> and <i>DIP2</i> transcripts after 45 min at 44°C.