The effect of vitamin-D3 on oxidative stress and the nuclear translocation of the glucocorticoid receptor.

<p>A: The ROS in airway epithelial cells was detected by confocal microscopy using a DCFH-DA probe. The ROS level was represented by fluorescence intensity (X400).B: The nuclear translocation of GR was assessed by immunocytochemistry of the airway epithelial cells treated with Dex for 30 minutes. FITC- conjugated anti-rabbit secondary Abs (X400). C: The PBMC in severe asthma exacerbation with V-D-deficiency or V-D-sufficiency were incubated with or without vitamin D3 for 12 hours. The nuclear translocation of GR was assessed by immunocytochemistry of the PBMC treated with Dex for 30 minutes. TRITC–conjugated anti-rabbit secondary Abs (X400).