Oxidative stress and DNA damage in severe asthma exacerbation.

<p>A: The intracelluar ROS levels in PBMCs from the normal controls, severe asthma exacerbation and V-D-deficiency (vitamin D3 <30 ng/ml) or V-D-sufficiency (vitamin D3>30 ng/ml) were detected by flow cytometry using DCFH-DA, which is oxidized to DCF in the presence of ROS. The ROS level was represented by the percentage of ROS positive cells. B: The intracellular ROS levels in PBMCs from these asthmatic participants were detected by a confocal microscope using a DHE probe. The ROS level in PBMCs from these asthmatic participants was represented by the fluorescence intensity (X400). C: The DNA damage in PBMCs from these asthmatic participants was detected by comet assay using a confocal microscope(X400). The DNA damage score was analyzed with <b>Comet Assay IV</b> software.