The change in FEV1%, ROS and DNA damage in severe asthma exacerbation treated with methylprednisolone and vitamin D3.

<p>A: The rates of the FEV1 change in severe asthma exacerbation treated with methylprednisolone and vitamin D3. B: The intracellular ROS in PBMCs from these asthmatic participants were detected by confocal microscopy using a DCFH-DA probe. The ROS levels were represented by the fluorescence intensity. The quantification of the ROS positive cell density in 10-random cell fields containing 500 cells(X400). C: The DNA damage in PBMCs from these asthmatic participants was detected by comet assay using confocal microscopy (X400). The DNA damage score was analyzed with <b>Comet Assay IV</b> software.