Importance of B7-1:CD28 axis in enhancing a Lass5 specific LnB5 T-cell clone activation.

<p>The RMA-S/pUB and RMA-S/B7-1 transfectants were used as targets recognized by a Lass5 specific LnB5 T-cell clone. Lass5 specific T-cell clone activation detected by the intracellular IFN-gamma release assays were conducted with stimulators RMA-S/pUB and RMA-S/B7-1 cells in (A) to (D). (A): 8×10³ T-cells were incubated with indicated amounts of RMA-S/pUB and RMA-S/B7-1 cells. (B): 8×10³ T-cells were incubated with 1×10⁵ stimulators that previously incubated with either anti-B7-1 mAb or isotype control (for RMA-S/B7-1). (C): 8×10³ T cells were incubated with either anti-CD28 mAb or isotype control before co-culture with 1×10⁵ stimulators (RMA-S/pUB or RMA-S/B7-1). (D): Before co-culture of the T-cells and stimulators, 8×10³ T-cells were incubated with either anti-CD28 mAb or Isotype control and 1×10⁵ RMA-S/B7-1 stimulator cells were incubated with either anti-B7-1 mAb or Isotype control. One out of at least two experiments with similar results was shown. <b>*</b> and ** indicated that P-values were less than 0.05.