Increase in Lass5 expression Bypasses B7-1/CD28 requirement for T effectors’ response.

<p>Lass5 specific LnB5 T-cell clone (A) and T-cell bulk culture (B) were used to determine B7-1/CD28 requirement. (A): Lass5 high expressing RMA-S/pUB.Trh4 and RMA-S/B7-1.Trh4 cells were used as targets that were recognized by LnB5 T-cell clone. The antibodies against CD80 (B7-1) or CD28 molecules were used to block B7-1/CD28 axis. The isotype Ig was used as a control. (B): Lass5-peptide (50 micromole) pulsed RMA-S/pUB and RMA-S/B7-1 cells were used as targets that were recognized by T-cell bulk culture for ⁵¹Cr-release assays. Pep means Lass5 peptide. One out of two experiments with similar results for each assay was shown. * * and *** indicated no statistical significance.