Experimental protocol. NC/Nga mice were given intranasal (i.n.) applications of a mixture (ADS airborne particles and/or Df, or CJ-1 soil and/or Df) as sensitization for 5 consecutive days (days 0–4).

<p>At 7 days after the last allergen sensitization, mice were challenged by allergen, followed by collection of BALF, lung tissue, and serum. Mice were divided into 6 groups: NS/NS, Df/Df, CJ-1/NS, CJ-1+Df/Df, ADS/NS, and ADS+Df/Df.