Endogenous NET23/STING expression correlates with levels of normal chromatin compaction observed in various cell types.

<p>(<b>A–E</b>) Transformed cell lines. (A) Western blot comparing levels of NET23/STING in different cell lines with α-tubulin used as a loading control. (<b>B</b>) Quantification of NET23/STING levels from three separate Western blots. The endogenous levels of NET23/STING have been corrected for α-tubulin levels and ordered from lowest to highest. (<b>C</b>) Using the algorithm described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111851#pone-0111851-g003" target="_blank"><b>Figure 3</b></a> to determine endogenous levels of chromatin compaction in the same cell lines, similarly ordered, reveals a general trend that cells with higher endogenous levels of NET23/STING have higher levels of chromatin compaction. (<b>D</b>) Table showing p-values for C, comparing all possible combinations using KS tests. (<b>E</b>) Nuclear size was also tested for each cell line, finding no notable differences. All p-values for nuclear size using KS tests were>0.05 with the exception of comparing HT1080 and EL-4 cells (p = 0.039) and HT1080 and Jurkat cells (p = 0.003). (<b>F–I</b>) Primary cell lines. (<b>F</b>) Basal NET23/STING protein levels for three primary cell lines relative to the AG line. (<b>G</b>) Cluster algorithm to determine endogenous levels of chromatin compaction based on DAPI staining. (<b>H</b>) P values for comparing cluster number between the different cell lines using KS tests comparing each to the others. (<b>I</b>) Nuclear size measured for the three primary lines to ensure that all were similar so that this parameter could not influence cluster number results.