Inhibition of Sirt1 in HUVECs induced premature senescence.

<p>(A) and (B) Profiles of <i>Sirt1</i> mRNA expression and Sirt1 protein expression 24 hours after transfection with Sirt1 siRNA. (C) and (D) Profiles of <i>PAI-1</i> mRNA expression and PAI-1 protein expression 72 hours after transfection with Sirt1 siRNA. (E) SA-β-gal staining was done 72 hours after Sirt1 siRNA transfection. *<i>P<0.05 vs</i> Mock group, n = 3. Mock, transfection reagent only group; NC, negative control siRNA group; siRNA, Sirt1 siRNA group.