<p><b>A) Caspase 3 activity</b> in cell lysates from adherent NPCs either left untreated or treated with 20 µM rotenone (R) for 18 hrs and then exposed to 1 uM staurosporine for 120 min before analysis. HTS analysis for caspase 3 activity from cell lysates was by activation of the fluorescent caspase substrate 7-amino-4-methylcoumarin (AMC) (Ex./Em. 340/440 nm) (n = 9, mean ± SEM, Ctrl/SNCA-Tri/SNCA-Tri KD, HG: 33/69/42, HG+R: 42/129/87, NG: 55/138/85, *p≤0.050, **p≤0.0035; from three independent experiments). <b>B)</b><b>Kinetics of caspase 3/7</b><b>activity</b> in permeabilized NPCs pretreated as described under B) and assayed 15 min after staurosporine treatment. Changes in caspase 3 activity are depicted as ΔµM AMC fluorescence/min + m...
<p>(A) F<sub>r</sub> values were determined for HeLa cells expressing EGFP<sup>C3</sup>Phanta and tr...
Apoptosis is an important phenomenon for investigating the efficacy of anti-cancer drug candidates. ...
<p>(A) Propidium iodide/Annexin V labeling assay in presence of caspase inibitor. The percentage of ...
<p>The exposure parameters and media are identified in the legend. Growth media were changed the sam...
<p>Percentage of ATP (representative of cell viability) in 293T-FF (black dots) and A549 (white dots...
<p>A) shows caspase 3/7 activity and B) cell lysis for U2OS cell line treated with increasing concen...
<p>Pan-caspase cleavage detection (ApoStat Apoptosis Detection Kit; R&D Systems) using flow cytometr...
<p>(A) Activation of caspase-3 was decreased in TCERG1-knockdown Jurkat cells treated with staurospo...
Caspase-3 is a key mediator of apoptosis in mammalian cells. Cells expressing caspase-3 substrate pe...
<p>All cell lines were incubated in 96-well plates for 48 h in culture medium supplemented with 100 ...
<p>A549 cells were treated with 1 μM of Staurosporine (STS), 10 μM of x-hederin (Hed) and 5, 10, and...
<p>(a) Caspase-3 cleavage after Staurosporine treatment: The left panel shows Caspase-3 cleavage in ...
<p>PC3 cells were seeded at 10<sup>6</sup> cells/ml (2 ml) in a 6-well plate overnight and then trea...
<p>Cells were treated with siRNAs for 72h after which cell viability and caspase activity were measu...
<p>Transfected MDA-MB-231 cells were treated with staurosporin (1 μM) for 5 h or with a mixture of a...
<p>(A) F<sub>r</sub> values were determined for HeLa cells expressing EGFP<sup>C3</sup>Phanta and tr...
Apoptosis is an important phenomenon for investigating the efficacy of anti-cancer drug candidates. ...
<p>(A) Propidium iodide/Annexin V labeling assay in presence of caspase inibitor. The percentage of ...
<p>The exposure parameters and media are identified in the legend. Growth media were changed the sam...
<p>Percentage of ATP (representative of cell viability) in 293T-FF (black dots) and A549 (white dots...
<p>A) shows caspase 3/7 activity and B) cell lysis for U2OS cell line treated with increasing concen...
<p>Pan-caspase cleavage detection (ApoStat Apoptosis Detection Kit; R&D Systems) using flow cytometr...
<p>(A) Activation of caspase-3 was decreased in TCERG1-knockdown Jurkat cells treated with staurospo...
Caspase-3 is a key mediator of apoptosis in mammalian cells. Cells expressing caspase-3 substrate pe...
<p>All cell lines were incubated in 96-well plates for 48 h in culture medium supplemented with 100 ...
<p>A549 cells were treated with 1 μM of Staurosporine (STS), 10 μM of x-hederin (Hed) and 5, 10, and...
<p>(a) Caspase-3 cleavage after Staurosporine treatment: The left panel shows Caspase-3 cleavage in ...
<p>PC3 cells were seeded at 10<sup>6</sup> cells/ml (2 ml) in a 6-well plate overnight and then trea...
<p>Cells were treated with siRNAs for 72h after which cell viability and caspase activity were measu...
<p>Transfected MDA-MB-231 cells were treated with staurosporin (1 μM) for 5 h or with a mixture of a...
<p>(A) F<sub>r</sub> values were determined for HeLa cells expressing EGFP<sup>C3</sup>Phanta and tr...
Apoptosis is an important phenomenon for investigating the efficacy of anti-cancer drug candidates. ...
<p>(A) Propidium iodide/Annexin V labeling assay in presence of caspase inibitor. The percentage of ...