MS based analysis of PTMs in HBZ.

<p>(A) MS/MS fragmentation of HBZ peptide DGLLSLEEELK*, where Lys is methylated. (B) MS/MS Fragmentation of HBZ peptide GPPGEK*APPR, where Lys is acetylated. (C) MS/MS Fragmentation of HBZ peptide DGLLS*LEEESR, where Ser is phosphorylated. (D) Summary of MS results. The HBZ sequence is provided, with covered sequences underlined. PTMs found are highlighted in the sequence and marked relative to the three domains of HBZ in a cartoon.