Effect of a panel of IFN inhibitors on BUNΔNSs virus growth in A549 cells.

<p>The inhibitor panel consists of small molecules that target the IKK2 (TPCA-1) and TBK1 (BX795, MRT6884, MRT6707) components of the IFN induction pathway and JAK1 (Cyt387, AZD1480, Ruxolitinib, Tofacitinib) a component of the IFN signaling pathway. Effect of the inhibitors was compared with A549 cells constitutively expressing viral IFN antagonists that block IFN production (BVDV-Npro) or IFN signaling (PIV5-V). (a) Effect of various inhibitor concentrations on plaque size formation. Plaques were visualized 2 days post-infection by crystal violet staining. (b) Virus growth monitored over time in presence of 2 µM inhibitor or the equivalent volume of DMSO and pfu/ml determined from reserved supernatants by titration in Vero cells.