Comparison of the time course of expression of SSAT and SMO in the kidneys of sham operated and injured wt- and PT-SSAT-Cko-mice.

<p>a) Kidney RNA (30 µg/well) from sham operated and injured wt- and PT-SSAT-Cko-mice (n = 2) was size fractionated and subjected to northern blot analysis in order to compare the expression levels of SSAT transcripts. The intensity of SSAT mRNA and 18s rRNA bands were determined by densitometry. The intensity of SSAT bands were normalized against that of the corresponding 18s rRNA bands. The graph depicts the average normalized SSATmRNA/18s rRNA values. b) Kidney RNA (30 µg/well) from sham operated (n = 1) and injured wt and PT-SSAT-Cko mice (n = 3) was size fractionated and subjected to northern blot analysis in order to compare the SMO mRNA levels.