Characterization of proximal tubule cell specific SSAT knockout mice.

<p>a) Disruption of the SSAT gene was confirmed by examining the PCR amplification products of genomic DNA from the tail (bottom panel) and kidney (top panel) of SSAT-Cko/Vill-Cre (PT-SSAT-Cko) and their Cre-deficient (SSAT-Cko) littermates. b) Kidney RNA (30 µg/well) from PT-SSAT-Cko and wt mice was size fractionated and subjected to northern blot analysis in order to assess the effect of cre mediated ablation of the SSAT gene on the expression of its mRNA.