Recapitulation of Premature Senescence Phenotypes in Differentiated Cells from WS iPSCs.

<p>(A) Differentiation of EBs from normal (TIG-3) and WS (WSCU01 #02 and #13) iPSCs. Differentiated cells from WS iPSCs showed premature senescence. SA-β-gal staining was performed on day 25 of differentiation. Bars = 100 µm. (B) Percentage of senescent cells after 25 days of differentiation. SA-β-gal-positive cells were counted in three randomly selected fields with 40× magnification. Values represent means of the three fields ± SD. (C) Expression of hTERT and p21 mRNAs in undifferentiated iPSCs (“U,” red columns), EBs after 12 days of formation (“E,” green columns), and differentiated cells after 25 days of differentiation (“D,” blue columns). Values represent means of three technical replicates ± SD. (D) Expression of SASP genes in differentiated cells from normal (TIG-3) and WS (WSCU01 #02 and #13) iPSCs after 25 days of differentiation. Graphs shows fold changes relative to undifferentiated iPSCs. Values represent means of three technical replicates ± SD.