Sustained ESC-like characteristics of WS iPSCs after Long-Term Culture.

<p>(A) Expression of pluripotency genes in A0031-derived WS iPSC clones in early and late passages. (B) Expression of hESC markers in A0031-derived WS iPSC clone #23 in early and late passages. Bars = 100 µm. (C) EB formation in A0031-derived WS iPSC clones from early and late passages. Bars = 100 µm. (D) Immunocytochemical analysis of differentiation of EBs into 3 germ layers for A0031-derived iPSC clone #23 in early and late passages. β-III tubulin (ectoderm), desmin (mesoderm), vimentin (mesoderm and parietal endoderm), and α-fetoprotein (Afp, endoderm). Bars = 100 µm. (E) Hematoxylin and eosin histology of teratomas from A0031-derived iPSC clone #23. Formation of all 3 germ layers is shown including melanin-producing cells (ectoderm), cartilage (mesoderm), and tracheal epithelium (endoderm).