TNF enhances MMP-9 production in CL patients.

<p><i>A</i>, PBMC from healthy subjects (HS) <i>(n = 12)</i> and CL patients (<i>n = 19)</i> were cultured in the presence or absence of SLA (5 µg/ml) for 72 hours, and TNF concentrations were determined by ELISA on supernatants. <i>B</i>, PBMC from HS (<i>n = 5</i>) were cultured in the presence or absence of recombinant TNF (5 ng/ml) for 24 hours and the levels of MMP-9 were determined by ELISA. <i>C</i>, PBMC from HS <i>(n = 6)</i> and CL patients <i>(n = 6)</i> was cultured in presence or absence of anti-TNF antibodies (10 µg/ml) for 24 hours. The levels of MMP-9 were determined by ELISA on supernatants. *p<0.05; ***p<0.0005.