Distribution of fatty acids epoxides and vicinal diols.

<p>The per-phospholipid concentrations of epoxides as AA-derived EpETrEs (<b>A</b>), EPA-derived EpETEs (<b>B</b>), DHA-derived EpDPEs (<b>B</b>) AA-derived DiHETrEs (<b>C</b>), EPA-derived Di HETEs (<b>D</b>), and DHA-derived DiHDPAs (<b>D</b>) are shown in lipoproteins and plasma. Regioisomers are color coded by our best estimate of homologous double-bond positions across all figures. Epoxides are products of CYP-epoxygenase and can also be generated via autooxidation. Vicinal diols are the unique products of soluble epoxide hydrolase on epoxides. The effect of P-OM3 adjusted for age and sex was uniform regardless of regioisomer. The mean adjusted effects are shown graphically in Figure S2 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111471#pone.0111471.s002" target="_blank">File S1</a>; A mixed model ANOVA was used to test for differences on ln[nM oxylipin/mM PL]. The least-squares mean [95% CI] are shown. Tukey's HSD test was used for post hoc differences between regioisomer levels, and regioisomers sharing a letter are not different. Since no interactions were significant, the term was dropped and the parameter estimate was used to calculate a mean effect [95% CI]. Note that since the test was on log-transformed data, the effect is a proportional one (<i>i.e.</i> percent change). Note the log scale of the <i>y</i>-axis. Note that EPA and DHA oxylipins are shown on the same graph for brevity, but constitute separate ANOVA tests.