Inactivation of GSTP1-1 by human defensins (HNP-1 and HNP-2).

<p>GSTP1-1 (90 pmoles) was incubated at 25°C with HNP-1 (0.54 nmoles) (open triangle), HNP-2 (open diamond) (0.54 nmoles) and both HNP-1 0.27 nmoles and HNP-2 (0.27 nmoles) (open square) in 70 µl (final volume) of potassium phosphate buffer, pH 7.0 (these defensin levels reproduce the average concentration of these proteins in saliva). An identical amount of GSTP1-1 was also incubated with 70 µl saliva (open circle). Each experiment was performed in triplicate (i.e. three different spectrophotometric determinations on the same sample). Error bars represent SEM.