Physiological assay of <i>GhSnRK2</i> gene silenced plants.

<p>(A) Phenotype of drought stressed plants. Water was withheld from <i>GhSnRK2</i> gene silenced and non-silenced plants for 5 days approximately two weeks post-inoculation. Each of the ten treated groups consisted of five plants. A photograph of each group representative was captured. (B) The survival rate was determined by withholding water from <i>GhSnRK2</i> gene silenced and non-silenced plants for 5 days approximately two weeks post-inoculation, and the survival rate was recorded as the percentage of plants that survived after re-watering for 3 days. (C) The relative water loss was determined two weeks after inoculation. The reduction in the fresh weight from the initial weight was determined at the indicated time and represented as the percentage of water loss. The experiment was replicated three times. (D) The mean RWC was analyzed after immersing the fresh detached leaves in distilled water for 4 h and oven drying at 80°C for 48 h. The values are presented as the means of three biological replicates. Asterisk denotes a significant difference (P<0.05). (E) The change in the chlorophyll content of <i>GhSnRK2</i> gene silenced and non-silenced plants under salt stress. (F) The phenotype of <i>GhSnRK2</i> gene silenced and non-silenced plants under salt stress.