Seed germination of the WT and <i>GhSnRK2</i> plants subjected to NaCl and exogenous ABA treatment and biomass accumulation of these plants.

<p>(A) Seed germination frequency of the WT and <i>GhSnRK2</i> transgenic plants cultured on MS medium supplemented with different concentrations of NaCl (50 mM or 100 mM) or ABA (0 µM, 0.3 µM, or 0.5 µM). (B) The survival rate of the WT and <i>GhSnRK2</i> transgenic plants cultured in MS medium containing 50 mM or100 mMNaCl. The mean values were compared using Student's T-test (p<0.05). (C) The germination rate in MS medium supplemented with 0.3 µM or 0.5 µM ABA. The values are presented as the mean germination rates (%) of approximately 200 seeds. Asterisk denotes a significant difference (P<0.05). (D) Biomass accumulation of the <i>GhSnRK2</i> transgenic and WT plants. For dry weight biomass, the dry weight in the roots and shoots was recorded after drying in an oven to a constant weight at 70°C for 48 h. (E) Fresh weight biomass of <i>GhSnRK2</i> transgenic plants and corresponding WT plants. The fresh weight of the roots and shoots was measured immediately after harvesting. Each of the three biological replicates consisted of 12 plants. Student's T-test was performed. Asterisk denotes a significant difference (P<0.05).