Effect of MDA5 knockdown on poly(I:C)-induced antiviral activity.

<p>Japanese flounder were administered with PBS (control), siRC2, siMDA5, poly(I:C), or poly(I:C) in the presence siRC2 or siMDA5. The fish were then infected with megalocytivirus, and the amount of virus in kidney (A) and spleen (B) was determined at different days (d) after infection. Data are expressed as the mean ± SE (<i>N</i> = 3). <i>N</i>, the number of experimental repeat. *<i>P</i><0.05; **<i>P</i><0.01.