Effect of MDA5 knockdown on poly(I:C)-induced gene expression.

<p>Japanese flounder were administered with PBS (control), siRC2, siMDA5, poly(I:C), or poly(I:C) in the presence siRC2 or siMDA5. The expression of immune genes in spleen was then examined by quantitative real time RT-PCR. Data are presented as means ± SE (<i>N</i> = 3). <i>N</i>, the number of experimental repeat. *<i>P</i><0.05; **<i>P</i><0.01.