Gene expression by RT-qPCR.
- Raquel Ordóñez (660111)
- Gabriel Gallo-Oller (660112)
- Soledad Martínez-Soto (660113)
- Sheila Legarra (660114)
- Noémie Pata-Merci (660115)
- Justine Guegan (5661436)
- Giselle Danglot (660116)
- Alain Bernheim (9135)
- Bárbara Meléndez (5661439)
- Juan A. Rey (420331)
- Javier S. Castresana (408962)
Publication date
November 2014
DOIAbstract
<p>The graphs represent the 2<sup>−ΔΔCt</sup> values obtained by RT-qPCR for neurospheres. The dotted line indicates the 2<sup>−ΔΔCt</sup> control (cell line) value equal to 1. Significance against control: p<0.05 (*); p<0.01 (**); p<0.001 (***) and p<0.0001 (****). <b>Corroboration of expression array (A).</b> Both SK-N-DZ (▪) and SIMA (▴) CSC-like cells showed a similar expression profile. <b>Stem Cell Markers expression (B).</b> Results confirmed a high overexpression of CSC markers in SIMA cell line after the neurosphere formation assay.</p
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