<p>The enzyme activity was measured using the standard enzyme assay (0.1 M Tris-HCl buffer, pH 9.0) after 5 h incubation at different pH values. Squares – 0.03 M Na COOH<sub>3</sub> (4.0–6.0); circles - 0.05 M Tris-HCl (7.0–9.0); diamonds – 1 M DEA (8.0–10.3); down triangle - 0.1 M NaHCO<sub>3</sub> (9.0–11.0); up triangle - 0.1 M glycine (9.0–11.0) (n = 9, p≤0.05).</p
The first step of the inactivation of the enzyme D-amino acid oxidase (DAAO) from porcine kidney at ...
<p>Stock solutions of each enzyme were incubated at 37°C/pH 6.8 for up to 125 hours. At the indicate...
<p>Effect of (a) pH on enzyme activity, (b) pH on enzyme stability; pH 3.0 (♦), pH 4.0 (□), pH 5.0 (...
<p>(A) The enzyme activity was determined by monitoring the formation of tagatose from galactose at ...
<p>A range of temperature (A) and pH (B) conditions were examined using substrate Gly-pro-AMC. To de...
<p>The pH stability of uninv (a), uninv2 (b) and M-inv2 (c) were tested at 37°C. The protein was inc...
<p><b>A</b>) Effect of pH on OiPncC activity measured by the HPLC Assay. The buffers used were 50 mM...
<p>The enzymes were incubated in 0.1 M B&R buffer (at pH 3, 3.5 and 7) at 25°C, and their residual a...
<p>The buffers used were sodium acetate (<i>open circles</i>), potassium phosphate (<i>closed circle...
Stability was assessed in seven different stabilising solutions and a control (gel filtration buffer...
<p>Panels A & B show the optimal temperature at pH 6.0 (A) and the optimal pH at 60°C (B) for 24 hou...
<p>Enzyme activity was determined by measuring the acetolactate-formation activity of each enzyme af...
<p>Knowpains were incubated at pH 5.5 (<b>A</b>) or pH 7.5 (<b>B</b>), identical aliquots were taken...
<p><b>A)</b> Thermal stability (blue dashed line) and activity (red solid line) with varying NaCl co...
<p>An amount of 0.1 g MCM-41-<i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL were dissolved in 10 mL Tris...
The first step of the inactivation of the enzyme D-amino acid oxidase (DAAO) from porcine kidney at ...
<p>Stock solutions of each enzyme were incubated at 37°C/pH 6.8 for up to 125 hours. At the indicate...
<p>Effect of (a) pH on enzyme activity, (b) pH on enzyme stability; pH 3.0 (♦), pH 4.0 (□), pH 5.0 (...
<p>(A) The enzyme activity was determined by monitoring the formation of tagatose from galactose at ...
<p>A range of temperature (A) and pH (B) conditions were examined using substrate Gly-pro-AMC. To de...
<p>The pH stability of uninv (a), uninv2 (b) and M-inv2 (c) were tested at 37°C. The protein was inc...
<p><b>A</b>) Effect of pH on OiPncC activity measured by the HPLC Assay. The buffers used were 50 mM...
<p>The enzymes were incubated in 0.1 M B&R buffer (at pH 3, 3.5 and 7) at 25°C, and their residual a...
<p>The buffers used were sodium acetate (<i>open circles</i>), potassium phosphate (<i>closed circle...
Stability was assessed in seven different stabilising solutions and a control (gel filtration buffer...
<p>Panels A & B show the optimal temperature at pH 6.0 (A) and the optimal pH at 60°C (B) for 24 hou...
<p>Enzyme activity was determined by measuring the acetolactate-formation activity of each enzyme af...
<p>Knowpains were incubated at pH 5.5 (<b>A</b>) or pH 7.5 (<b>B</b>), identical aliquots were taken...
<p><b>A)</b> Thermal stability (blue dashed line) and activity (red solid line) with varying NaCl co...
<p>An amount of 0.1 g MCM-41-<i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL were dissolved in 10 mL Tris...
The first step of the inactivation of the enzyme D-amino acid oxidase (DAAO) from porcine kidney at ...
<p>Stock solutions of each enzyme were incubated at 37°C/pH 6.8 for up to 125 hours. At the indicate...
<p>Effect of (a) pH on enzyme activity, (b) pH on enzyme stability; pH 3.0 (♦), pH 4.0 (□), pH 5.0 (...