YB-1 interacts with IL-6 mRNA but does not affect IL-6 mRNA stability.

<p>(<b>A</b>) Macrophages were exposed to LPS (80 ng/ml) and then subjected to RIP assay using appropriate primer pairs (P4, P6, P8, and P9 in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112754#pone.0112754.s002" target="_blank">Table S1</a></b>). YB-1 bound IL-6 mRNA, but not TNF-α mRNA. (<b>B</b>) The effect of YB-1 depletion on IL-6 or TNF-α mRNA stability in LPS-stimulated macrophages. Total mRNA was isolated at different time points after actinomycin D (6 µg/ml) treatment and each RT-PCR was analyzed by the indicated primer pairs (P4, P5, and P8). (<b>C</b>) Each graph represents densitometric analysis of RT-PCR data. Relative densitometric values for each mRNA were corrected by dividing each value by that for the GAPDH mRNA in each blot. *<i>P</i><0.001 (student's t-test). Data are representative of at least three independent experiments.