Various evidences of cell death induced by different forms of silver materials.

<p>(A) The Sub-G1 DNA analysis for PMA-primed THP-1 cells after treatment with silver materials for 24 h. The extent of the sub-G1 peak was determined by flow cytometry using PI staining. Data are represented as the mean ±SD (n = 3). (B) The decrease in the extent of relative cell size as measured using forward scattering of flow cytometry. PMA-primed THP-1 cells were treated with different forms of silver materials for 24 h. Data are represented as the mean ±SD (n = 3). (C) Annexin-V staining of PMA-primed THP-1 cells to detect the mode of cell death in the presence of different forms of silver materials for 6 h. AnnexinV/PI analysis was performed to assess the percentage of viable, apoptotic, and necrotic cells.