Spectroscopic characterization of bicarbonate stable heme aggregates formed in presence of BNT1/BNTM.

<p>Panels A, C and E show UV-visible spectra of β-hematin and BNT1/BNTM mediated bicarbonate stable heme aggregates respectively. These aggregates were incubated overnight in 2.5% aqueous SDS prior to recording spectra (blue line). Note the broad Soret (400 nm) and the weak but characteristic peak at 655 nm. Typical hematin spectra (sharp Soret at 400 nm - red line) were obtained when each suspension was made 0.02 N with respect to NaOH. Panels B, D and F show the FTIR spectra of β-hematin and BNT1/BNTM mediated bicarbonate stable heme aggregates respectively. Peaks at 1664 cm⁻¹ and 1210 cm⁻¹ in FTIR spectrum of heme aggregates mediated by BNT1 and BNTM confirm their identity as β-hematin.