Clonogenic analysis with the combination of the PI3K/mTORi, PF-04691502 (PF-502) combined with the MEKi, PD-0325901 (PD-901) on four colorectal cancer cell lines.

<p>(A) LOVO, (B) HCT116, (C) WIDR, and (D) GEO were plated in 6 well plates and exposed to single agent the PI3K/mTORi, PF-04691502, the MEKi, PD-0325901 or the combination for 72 hours. Drug was removed and replaced with media to allow for regrowth of clones. Cells were then stained with crystal violet and photographed. (E) The crystal violet was solubilized in 1% acetic acid and the absorbance was assessed on a plate reader. The data was normalized to control and graphed. All data presented as mean±SD, ANOVA Tukey’s adjusted p values: *<i>p</i>,0.05, **<i>p</i><0.01, ***<i>p</i><0.001 vs vehicle, #<i>p</i>,0.05, ##<i>p</i><0.01, ###<i>p</i><0.001 vs PF-502, &<i>p</i>,0.05, &&<i>p</i><0.01, &&&<i>p</i><0.001 vs PD-901. All data represents three independent experiments.