Proliferative effects on colorectal cancer cell lines plotted as IC₅₀ following exposure to the MEKi, PD-0325901 (0–1 umol/L).

<p>Cells were plated in 96 well plates and allowed to adhere for 24 hours. Cells were exposed to increasing concentrations of compound for 72 hours and proliferation was assessed using SRB assay. Proliferation assays were conducted in triplicate and IC₅₀ values were calculated from the average proliferation. Mutational status of KRAS, BRAF, and PIK3CA are in colored boxes.