Dopamine transporter (DAT) is present in equine ejaculated sperm.

<p>The presence of DAT in ejaculated equine sperm was investigated with immunodetection and microscopy methods. <b>A)</b> Sperm were fixed and analyzed by immunofluorescence using an anti-DAT antibody or only secondary antibody as negative control. <b>B)</b> Magnification of DAT immunodetection in sperm. Bar scale is 10 µm. <b>C)</b> 80 µg of total protein extract from rat brain (line 1) and equine sperm (line 2) were analyzed with SDS-PAGE and Western blot using a specific human anti-DAT antibody. Images are representative of 3 independent experiments. <b>D)</b> UREA/SDS-PAGE and Western blot analysis for total protein extract (100 µg) for rat brain (line 1 and 3) and equine sperm protein extract (line 2 and 4), with (line 2 and 4) or without (Line 1 and 3) anti-Dat antibody preabsorbed by immunogenic peptide (SC-7515 P).