Expression of Wnt5a suppresses tumor formation and increases latency in MMTV-Wnt1 mice.

<p>(A) Frequency of tumor formation in 6-month-old MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a mice (<i>P</i>-value = 0.001). (B) Kaplan-Meier-type curve to measure tumor latency. MMTV-Wnt1;MMTV-Wnt5a mice display a 6- to 7-week delay in tumor onset (<i>P</i>-value = 0.0045; n = 22 MMTV-Wnt1, n = 25 MMTV-Wnt1;MMTV-Wnt5a). (C–E) <i>Tumor proliferation</i>. Sections from MMTV-Wnt1 (C) and MMTV-Wnt1;MMTV-Wnt5a (D) mammary tumors stained with anti-Ki-67 antibody (Ki-67, green; nuclei, blue). No statistical difference (T-test) of Ki-67 staining was observed (E). Values are shown as means +/− standard error (n = 6 MMTV-Wnt1 tumors, 3 fields per tumor; n = 5 MMTV-Wnt1;MMTV-Wnt5a tumors, 3 fields per tumor).