Suppression of LC3-II accumulation by rTSST-1 in the cells is superantigenic activity-independent.

<p>Autophagy in HeLa 229 cells was induced by nutrient-starvation (KRB) with or without lysosomal protease inhibitors and 10 µg/ml rTSST-1, mTSST-1, SEA, SEB or SEC. Cells in MEM were used control. At 4 h of induction, LC3-II was detected by Western blotting (A) and the intensity of LC3-II band was quantified (B) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113018#pone-0113018-g005" target="_blank">Figure 5C</a>. The data is provided as SD of at least 3-independent experiments.