EGFP-HSP70 zebrafish exposed to tBHQ.

<p><b>A.</b> Embryos from hsp70-EGFP transgenic zebrafish were exposed to DMSO (0.1%) or tBHQ (10 µM) for 4 hrs at 1-dpf. After exposure, embryos were washed and held for 4 additional hours prior to fluorescence microscopy for detecting EGFP expression. <b>B.</b> Another experiment was performed by exposing 4-dpf eleutheroembryos (n = 15) to DMSO (0.1%) or tBHQ (10 µM) for 4 hrs at 28°C. After exposure, eleutheroembryos were washed with 0.3× Danieau's and inspected 4 hrs post-exposure for EGFP expression by fluorescence microscopy.