Reactivity of sera of mice injected with <i>T</i>. <i>denticola</i> strains to cellular components of <i>T</i>. <i>denticola</i>.

<p>The whole-cell lysate of wild-type <i>T</i>. <i>denticola</i> was applied to SDS-PAGE (11% gel) and blotted on a PVDF membrane. The PVDF membrane was cut into strips and reacted with each serum sample collected at 3 weeks after injection. As a negative control, sera of mice injected with PBS were pooled and subjected to immunoreaction. Numbers denote individual mice. Major bands were indicated by a, b, c, and d and were predicted to react to TDE1072, OppA, Msp, and TmpC, respectively. A molecular-weight standard (kDa) is shown at the left.