TAT-survivin (T34A) expression in <i>E. coli</i> BL21 (DE3) under acetate stress in shake flasks and bioreactors.

<p>A) and B), TAT-survivin (T34A) expression and cell growth of <i>E. coli</i> BL21 (DE3) under different conditions of acetate stress in shake flasks. The experiments were conducted in 50 mL of YTA-glucose medium (pH 7.5). C) and D), The profiles of TAT-survivin (T34A) expression, cell growth and acetate production in a 30-L bioreactor. The strategy of maintaining glucose at 10±5 g/L led to a constant increase of medium acetate from 10 h. E) The DO profile of the 30-L bioreactor cultivation from 10 h when IPTG was added (acetate: 110–250 mM). F) The DO profile of the 4-L bioreactor cultivation from 4 h when IPTG was added (acetate: 5–30 mM). In both bioreactors <i>E. coli</i> BL21 (DE3) cells were cultivated in YTA medium, but induced at different levels of acetate.