Expression patterns of C/EBPα, C/EBPβ, and C/EBPδ in human squamous cell carcinoma (SCC).

<p>(<b>A</b>) Frozen skin sections from patients with SCC were fluorescently immunostained, using antibodies to C/EBPα, C/EBPβ, or C/EBPδ (as indicated on left) followed by a Cy3-fluorotagged anti-IgG secondary antibody. Hematoxylin and eosin (H&E) stains were done on parallel sections. Tumor nests (<i>n</i>), and normal hair follicle epithelium <i>(ep)</i> and cortex <i>(C)</i>, are indicated. <i>Inset: Arrowheads</i> indicate well-differentiated squamous cells, expressing high levels of keratins (<i>red</i>). <i>Scale bars</i>, 50 µm. (<b>B</b>) Quantification of fluorescent staining intensities for the three C/EBP family proteins (α,β and δ) in eleven SCC tumors, relative to normal follicles; mean ± SD.