Enhanced intestinal ME1 expression with HF diet induces jejunum lipogenic- and proliferation-associated gene expression.

<p>A) Relative expression of jejunum genes of WT and ME1-Tg mice [Exp. 2 (n = 7−9/group)]. B) Western blot and corresponding band densitometry analysis of FASN in the jejunum of WT and ME1-Tg mice (Exp. 2). C–E) Effects of ME1 over-expression on intestinal epithelial cell proliferation and gene expression <i>in vitro</i>. Rat intestinal epithelial cells (IEC-6) were transfected with control or hME1 expression vectors; overexpression of human ME1 mRNA, but not beta-actin mRNA, was observed by RT-PCR (C). At 48 h, cells were evaluated for proliferation by MTT assay (D), and at 96 h (E) evaluated for expression of <i>Fasn</i>, <i>Scd1</i>, <i>Rxrg</i> and <i>Lpl</i> genes (n = 3 replicates/group). F) Gene expression of <i>Fasn</i>, <i>Angptl4</i>, <i>Lpl</i>, <i>Fgr</i>, <i>Irs1</i> and <i>Irs2</i> in jejunums of WT and MOD-1 mice fed HF diet (n = 5/group). RT-PCR was repeated twice in all experiments, and MTT proliferation assay was repeated thrice. Bar graphs represent mean ± SEM. Significant differences at *<i>P</i><0.05 and ** <i>P</i><0.01 between genotypes.