Integrin β4, αV, α5 and β3, were differentially regulated among the three cell lines in response to glucose.

<p>(A) Integrin β4 was weakly expressed, or undetectable in the MDA-MB-231BO and MDA-MB-231BR cells respectively however, it was expressed in all glycemic conditions in MDA-MB-231P cells which was significantly higher than the variants cells p>0.001 (graph on the right). Integrin αv was down regulated in MDA-MB-231BO and not detected when cells were exposed to DMEM 0 mM glucose (B0) (graph on far right). Integrin α-V was significantly decreased under hypoglycemia only in MDA-MB-231P cells p = 0.001. Integrin α5 was expressed in all the three cell lines and was down regulated in the absence of glucose in all the cells in study (bottom graph on the right and lower left panel). Integrin β3 was not detectable in the MDA-MB-231BO cells, and barely detectable in the MDA-MB-231P cells. It was expressed in detectable levels in MDA-MB-231BR cells and was slightly down regulated in reduced glucose. Error bars represent ± standard deviation. Note: β-Actin was used in all the blots as a protein loading control.