ING1b regulates miR-371-5p expression.

<p>(A) The expression of miR-371-5p in Panc-1 cells in response to ING1 overexpression for 48 hs, was determined by quantitative real-time PCR assays (upper panel. Bars represent the mean ± SD (**<i>P</i><0.01, n = 5). (Lower panel) Western blot was performed to analyze ING1 expression. (B) miR-371-5p level was determined using real-time RT-PCR in Panc-1 cells in response to treatment with the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA). Bars represent the mean ± SD (upper panel, *<i>P</i><0.05, n = 5). (Lower panel) Western blot was performed to analyze ING1 expression. (C) Cells infected with control GFP adenovirus or with the same virus also encoding ING1 were harvested 24 h later and prepared for ChIP analysis using control or ING1 antibodies. PCR of immunoprecipitation products showed that ING1 bound to the upstream region of miR-371-5p.