Effect of IE1_CORE on the integrity of PML-NBs and the SUMOylation of PML.

<p>(A) Dispersal of PML requires the IE1_IDRc. HFF cells were transiently transfected with expression plasmids encoding FLAG-tagged full-length hIE1(1–491) or truncated hIE1 variants followed by immunodetection of hIE1 proteins using an anti-FLAG antibody and of endogenous PML. (B) DeSUMOylation of PML by IE1_CORE. HEK293T cells were cotransfected with expression plasmids encoding FLAG-PML (isoform VI), FLAG-hIE1 variants as indicated and Myc-SUMO3. PML (upper panel), SUMOylated proteins (second panel), IE1 (third panel) and β-actin (lower panel) were detected by Western blot analysis. (C) Release of Sp100, hDaxx and ATRX from PML foci by IE1_CORE. The hIE1_CORE(1–382) was stably expressed in HFFs followed by immunodetection of hIE1, PML, Sp100, hDaxx or ATRX by triple staining as indicated.