Western blot analysis of mAbs raised against the homologous DENV serotype.

<p>Purified gamma-irradiated DENV-1 BR/01-MR (A), DENV-2 BR/01-01 (B), and DENV-3 290-02 (C) were subjected to 13% SDS-PAGE and electroblotted onto nitrocellulose membranes. Proteins were stained with the mAbs, followed by anti-mouse IgG conjugated to alkaline phosphatase. The flavivirus-specific mAb 4G2 and a non-correlated mAb that binds to hantavirus nucleoprotein (clone 572/7A) were used as positive (+) and negative (−) controls, respectively.