Representation of the reactivities of major groups of monoclonal antibodies.

<p>Indirect immunofluorescence of C6/36 cells uninfected (MOCK) or infected with DENV-1 (BR/90), DENV-2 (ICC 266), DENV-3 (290-02) and DENV-4 (TVP 360) isolates. Cells were fixed in methanol:acetone and stained with different mAbs, followed by Alexa-Fluor 488-conjugated anti-mouse immunoglobulin. Monoclonal antibody 4G2 and a non-correlated anti-hantavirus mAb (clone 572/7A) were used as positive and negative controls, respectively. Distinct groups of mAbs were raised against DENV: 1) group-specific (D3 424/8G); 2) subcomplex-specific (Anti-DENV-1, anti-DENV-3 and anti-DENV-4; clone D1 463/G6/H2); and 3) serotype-specific (anti-DENV-3 D3 290/4C/G9) mAbs. Images were produced in a Leica AF6000 Modular System. Scale bars are 30 µm.