MIF^−/− bone marrow macrophages have reduced inflammatory signature compared to WT mice.

<p>Levels of pro-inflammatory (a) IL-6, (b) IL-1β (c) MCP-1 secretion into media from lean WT and MIF^−/− BMM stimulated ±LPS. (d) Gene expression analysis of <i>Il-6</i> in WT and MIF^−/− BMM±LPS, n = 5/group. (e) Immunoblot analysis of phosphorylated and whole cell ERK, JNK, p38, NFkB and β-actin stimulated ±LPS, n = 3/group. WT mice represented by white bars, MIF^−/− mice represented by black bars in all graphs. Data are mean ± SEM, *p&lt;0.05, **p&lt;0.01 and ***p&lt;0.001 w.r.t WT+LPS.