Western blot analysis of Raf, Erk, Jnk, and Akt phosphorylation in EL4 cells.

<p>EL4 T cells were incubated in DMEM medium with an immobilized anti-CD3/CD28 mAb in the presence or absence of si-OCILRP2. After incubation at 37°C for 48 h, the T cells were lysed, and aliquots of 20 µg of the whole-cell lysate were analyzed by western blotting using antibodies against phospho-Erk, Erk, phospho-Jnk, and Jnk (a) and phospho-Raf and phosphor-Akt (b). The data are expressed as a percentage of the level of active Erk or Jnk of the total Erk or Jnk. Each data point represents the mean ± S.E. from three separate experiments.