Effects of <i>EGR4</i> gene on cell growth.

<p>A: Expression of <i>EGR4</i> in SCLC and NSCLC cell lines was determined by semi-quantitative RT-PCR. B: Effects of <i>EGR4</i> knockdown on cell proliferation in SBC-5 cells. Real-time PCR of <i>EGR4</i> in siEGFP- or siEGR4 (siEGR4-1, siEGR4-2)-treated cells at 5 days after siRNA treatment (n = 2, *<i>P</i><0.05). <i>ACTB</i> was used as a quantitative control for real-time RT-PCR. C: Cell proliferation was determined by MTT assay at 5 days after siRNA treatment (n = 3, ***<i>P</i><0.005). (si-1; siEGR4-1, si-2; siEGR4-2). D: Growth–promoting effect of exogenous EGR4 on NIH3T3 cells (*<i>P</i><0.05, **<i>P</i><0.01, <i>NS</i>, no significance). Western blot analysis was performed at 96 h after transfection (left panel). MTT assay was performed at 48, 72 and 96 h after transfection with FLAG-EGR4 (black) or mock vector (white) (right panel). These experiments were performed in triplicate.