Flow cytometric analysis of B cell populations resident in SMGs.

<p>Flow cytometry was performed on cells prepared from SMGs from male <i>Myd88</i>^+/+ mice and <i>Myd88</i>^-/- mice at 10 weeks old. A: Analysis of SMG cells obtained from four mice for expression CD45 and B220 (30,000 cells each). The percentage and cell number of the cells within the outlined area in dot plots (indicative of CD45⁺B220⁺ cells; others shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113333#pone.0113333.s002" target="_blank">Figure S2A</a>) were shown. The data of percentage were graphically shown as means ± SD (n = 4 per group) and are representative of three independent experiments. **, <i>P</i><0.01 (unpaired Student's <i>t</i>-test). B: Analysis of SMG cells obtained from four mice for expression CD138 and B220 (30,000 cells each). The percentage and cell number of the cells within the outlined area in dot plots (indicative of CD138⁺B220⁺ cells; others shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113333#pone.0113333.s002" target="_blank">Figure S2B</a>) were shown. The data of percentage were graphically shown as means ± SD (n = 4 per group) and are representative of three independent experiments. **, <i>P</i><0.01 (unpaired Student's <i>t</i>-test). **, <i>P</i><0.01 (unpaired Student's <i>t</i>-test). C: Analysis of SMG cells obtained from four mice for expression CD138 and IgA (30,000 cells each). The percentage and cell number of the cells within the outlined area in dot plots (indicative of CD138⁺IgA⁺ cells; others not shown) were shown. The data of percentage were graphically shown as means ± SD (n = 4 per group) and are representative of three independent experiments. *, <i>P</i><0.05 (unpaired Student's <i>t</i>-test). D: Analysis of B-1 cells and B-2 cells in SMG cells obtained from four mice (30,000 cells each). For discrimination of B-1a cells in the dot plots, B220-positive cells were separated into CD5⁺ cells (B-1a cells) and CD5^- cells (B-1b plus B-2 cells). For discrimination of B-2 cells, B220-positive cells were separated into CD23⁺ cells (B-2 cells) and CD23^- cells (B-1 cells). The percentage and cell number within the outlined area in dot plots were shown. The data of percentage of B220⁺CD23^- B-1 cells were graphically shown as means ± SD (n = 4 per group) and are representative of three independent experiments. *, <i>P</i><0.05 (unpaired Student's <i>t</i>-test).