Induction of IL-8 cytokine production in Caco-2 cells by recombinant SpaFED-piliated lactococci.

<p>Caco-2 cells were treated with normalized cultures of recombinant WT (GRS1189) and SpaF pilin-deleted (GRS1126) SpaFED-piliated lactococci (MOI 100). GRS71 and GRS1052 cells (MOI 100) were used as controls. Endogenous IL-8 cytokine production levels in spent cell culture supernatants were measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113922#s4" target="_blank">Materials and Methods</a>. Triplicate measurements were taken for the experiments, which were repeated independently four times. SEM is displayed as error bars. Pairwise differences between GRS1189 and GRS1226 data are deemed significant (<i>P</i>≤0.05).