Chemical shift perturbation data for SGTA_NT/UBL interactions.

<p>A–D: Ribbon views coloured according to reciprocal chemical shift perturbation upon binding partner proteins. Residues whose shifts are greater than one standard deviation above the mean chemical shift are coloured darkest red. Those below the mean are coloured white and shifts between these points are graded pink. A) BAG6_UBL B) UBL4A_UBL C) SGTA_NT upon binding BAG6_UBL D) SGTA_NT upon binding UBL4A_UBL; E–F: Region of ¹H-¹⁵N HSQC spectra of ¹⁵N-labelled SGTA_NT before (black) and after (blue/maroon) titration with saturating quantities of unlabelled BAG_UBL (E) and UBL4A_UBL (F) Residue A28 splits upon binding to BAG6_UBL but not upon binding UBL4A_UBL. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113281#pone.0113281.s001" target="_blank">Figure S1</a> for full HSQC data on all split peaks.