Flow cytometry analysis of EpCAM positive cells from human primary breast tumors, xenografts, and breast cancer cell lines.

<p>Freshly harvested primary or xenograft tumor material was minced and digested and the whole cell suspension was stained with anti-EpCAM antibody combined with anti-CD24 and anti-SSEA-4. A) Flow analysis of EpCAM positive cells from five randomly chosen primary breast cancer tumors. F indicate tumor number, ER = estrogen-, PR = progesterone-, and Her2- receptor status are indicated under the corresponding dot plot. IDC indicates that primary tumor was invasive ductal carcinoma. B) Flow analysis of EpCAM positive cells from three PDX models and two breast cancer cell lines. The dot plots illustrate the distribution of CD24 and SSEA-4 expressing cells. Red dots are antibody stained cells; black dots represent unstained control.